Decolorization of Synthetic Dyes by Ficus carica Latex Peroxidase Isoenzymes

Aims: The current study aims to elucidate the potential of Ficus carica latex peroxidase isoenzymes for decolorizing different synthetic dyes in comparison to the commercial horseradish peroxidase. Study Design: The decolorization of 20 dyes was investigated using the purified F. carica latex peroxidase isoenzymes (purified FP1 and partially purified FP2, and FP3), and horseradish peroxidase (HRP) as a control. Place and Duration of Study: Molecular Biology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt, between January 2017 and March 2018. Methodology: The purified and partially fractions of peroxidase isolated from latex of F. carica were used for the present study. Stock solutions of the dyes were prepared in 0.05 M sodium acetate buffer (pH 5.5) and diluted to the requested concentrations ranged from 12 to 330 µM in order to get maximum absorbance does not exceed 1.5 as initial reading. The efficiency of decolorization was expressed in terms of percentage. All experiments were performed in triplicate. Results: F. carica latex peroxidase isoenzymes and commercial horseradish peroxidase were able to decolorize some of tested dyes and the extent of decolorization achieved with different dyes classes were varied according to different chemical structure of each dye. The decolorization efficiency after 3 h of incubation at 40°C using 6.4 U/ml of peroxidase activity of FP1, FP2, FP3 and HRP, was found to be extremely efficient in decolorizing some dyes and relatively low in other dyes. Conclusion: The efficiency of F. carica latex peroxidase isoenzymes toward different synthetic dyes meet the prerequisites needed for environmental and industrial applications.

Tratamiento simultáneo de anquilosis temporomandibular unilateral congénita y del microlaterognatismo asociado / Simultaneously treament of congenital unilateral temporomandibular ankylosis and consecutive microlatherognathism

Este artículo tiene como objetivo reportar los resultados obtenidos a corto y largo plazo, del tratamiento con enfoque multidisciplinario de una anquilosis unilateral congénita de la articulación temporomandibular asociada a un síndrome de malformación embrionaria, en un niño de 12 años de edad, en el que se utilizó un distractor externo bidimensional con un doble propósito: como fijador para mantener el espacio logrado con la artroplastia y como distractor para elongar la rama mandibular hipotrófica, activado 5 días después de la osteotomía, con el objetivo de eliminar la anquilosis y el microlaterognatismo mandíbular consecutivo de ella, simultaneamente de manera funcional y dinámica(AU)

The paper reports the short- and long-term results obtained from the multidisciplinary treatment of a congenital unilateral ankylosis of the temporomandibular joint associated to an embryonic malformation in a 12-year-old boy, using an external bidimensional distraction device with a two-fold purpose: as fixator to maintain the space achieved by arthroplasty, and as distractor to elongate the hypotrophic mandibular branch, activated 5 days after osteotomy, with the purpose of eliminating ankylosis and consecutive mandibular microlaterognatism, both functionally and dynamically(AU)

Congenital duodenal diaphragm in eight children

Congenital duodenal obstruction [CDO] is a common and usually easy to diagnose cause of intestinal obstruction in the newborn, except when the cause of the obstruction is a duodenal diaphragm. We describe our experience with eight children who had intrinsic duodenal obstruction secondary to a duodenal diaphragm. The medical records of 22 children with the diagnosis of congenital intrinsic duodenal obstruction were reviewed for age at diagnosis, sex, gestation, birth weight, clinical features, associated anomalies, method of diagnosis, treatment and outcome. Operative findings and procedures were obtained from the operative notes. Eight of the 22 children [36.4%] had congenital duodenal diaphragm [CDD]. In all children, the diagnosis was made from plain abdominal X-ray, which showed the classic double-bubble appearance, and barium meal, which showed duodenal obstruction. Four patients had associated anomalies, including two with Down's syndrome. Intraoperatively, five patients were found to have duodenal diaphragm with a central hole, while the other three had complete duodenal diaphragms. Postoperatively, all patients did well. Six required total parenteral nutrition. Conclusions: The 100% survival rate among these children is comparable to that in Western countries, and can be attributed to the lack of major associated abnormalities, good perioperative management, and the availability of total parenteral nutrition

Diagnostic value of bone marrow examination in haematological and non haematological diseases

In this study, 223 bone marrow biopsies and / or aspirations were performed. The main indications for bone marrow examination were diagnosis and management of acute leukaemia [45-20.2%], staging of lymphoma [29-13.1%], evaluation of pancytopenia [31-14.1%], thrombocytopenia [26-11.7%], anaemia [16-7. 2%], pyrexia of unknown origin [PUO] [14-6.1%], lymphadenopathy [16-7. 3%], hepatosplenomegaly [12-5.3%], to exclude metastasis [7-3.2%] and miscellaneous [25-11%]. The most common diagnosis encountered were: acute lymphoblastic leukaemia [35-15.7 %], idiopathic thrombocytopenic purpura [13-5.7%], acute myeloid leukaemia [12-5.7%], hyper8plenism [12-5.7%], chronic myeloid leukaemia [12-5.7%], megaloblastic anaemia [7-3.1%] and multiple myeloma [3-1.3%]. It was concluded that bone marrow examination is an important investigation for establishing the diagnosis in many conditions especially haematological neoplasms. The most common indication in this study was to confirm and manage acute leukaemia and the most common diagnosis was acute lymphoplastic leukaemia

Idiopathic thrombocytopenic purpura and helicobacter pylori

Helicobacter pylori [H. pylori] has been considered for many years as the aetiologic agent of gastritis, peptic ulcer, gastric cancer and mucosal-associated lymphoid tissue lymphoma. The prevalence of H. pylori infection and the effect of eradication in a series of 69 idiopathic thrombocytopenic purpura patients were investigated. H. pylori infection has been documented in 35 of 69 chronic ITP patients [50.7%] by 13C urea breath test and confirmed by histologic examination. There was a significant increase in the percentage of patients responding to corticosteroid therapy in uninfected group compared to infected patients with H. pylori [85 % vs 62.5%]. There was a significant increase in platelet count in all infected patients after eradication therapy for H. pylori infection. H. pylori eradication may provide a good option for treatment in some idiopathic thrombocytopenic purpura patients